Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TNIK Double Nickase Plasmid (h): sc-401523-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNIK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TNIK Double Nickase Plasmid (h) and TNIK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNIK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TNIK Antibody (C-1): sc-377215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNIK Double Nickase Plasmid (h) | sc-401523-NIC | 20 µg | $410.00 | |||
TNIK Double Nickase Plasmid (h2) | sc-401523-NIC-2 | 20 µg | $410.00 |
TNIK (TRAF2 and NCK interacting kinase) encodes a serine/threonine kinase of the germinal center kinase family that integrates signals controlling cytoskeletal organization, cell polarity, and transcriptional programs. TNIK functions as a regulator of Wnt/β-catenin signaling through interactions with TCF/LEF transcriptional complexes and contributes to pathways influencing adhesion, migration, and neuronal development. Dysregulated TNIK activity or expression has been associated with aberrant Wnt pathway output and altered proliferative or invasive phenotypes in multiple disease contexts, making it a useful node for mechanistic studies of signaling-network control. In human cells, TNIK is also studied for its roles in synaptic function and kinase-driven signaling cross-talk that shapes cellular state transitions.
TNIK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNIK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNIK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNIK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNIK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.