Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TIS11B Double Nickase Plasmid (h): sc-416672-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TIS11B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TIS11B Double Nickase Plasmid (h) and TIS11B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZFP36L1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TIS11B Antibody (1A3): sc-293267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIS11B Double Nickase Plasmid (h) | sc-416672-NIC | 20 µg | $410.00 | |||
TIS11B Double Nickase Plasmid (h2) | sc-416672-NIC-2 | 20 µg | $410.00 |
Human ZFP36L1 (TIS11B) encodes an AU-rich element–binding RNA-binding protein that promotes mRNA deadenylation and decay, thereby shaping transcript stability and translation. Through interactions with the CCR4–NOT deadenylase complex, TIS11B regulates post-transcriptional programs linked to immune signaling, hematopoietic differentiation, and cell-cycle control. ZFP36L1 activity integrates with cytokine-responsive pathways and immediate-early gene networks to constrain inflammatory and proliferative outputs. Dysregulated ZFP36L1-mediated mRNA turnover has been associated with aberrant lymphoid development and oncogenic transcriptional states, making it a useful node for studying RNA decay in disease-relevant contexts.
TIS11B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZFP36L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZFP36L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZFP36L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZFP36L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.