TIA-1 Antibody (G-11): sc-365349

TIA-1 Antibody (G-11) is a mouse monoclonal IgG1 antibody that detects TIA-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIA-1, a crucial type I transmembrane protein, is primarily located in the nucleus and plays a significant role in regulating apoptotic cell death, particularly in response to FAS signaling. This localization enables TIA-1 to participate in the post-transcriptional regulation of gene expression during apoptosis, influencing the fate of cells under stress or damage. Upon FAS ligation, TIA-1 rapidly translocates to the cytoplasm, where TIA-1 interacts with various signaling molecules, including the serine/threonine kinase FAST, which activates during this process. The phosphorylation state of TIA-1 is critical for function, as phosphorylation modulates RNA binding ability and regulates the expression of pro-apoptotic factors. Anti-TIA-1 antibody (G-11) serves as an essential tool for researchers studying apoptosis mechanisms and intricate signaling pathways involved in immune responses, providing insights into potential therapeutic targets for diseases characterized by dysregulated cell death.

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TIA-1 Antibody (G-11) References:

1. TIA-1 and TIAR activate splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on their own pre-mRNAs.  |  Le Guiner, C., et al. 2001. J Biol Chem. 276: 40638-46. PMID: 11514562
2. Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation.  |  Anderson, P. and Kedersha, N. 2002. Cell Stress Chaperones. 7: 213-21. PMID: 12380690
3. TIA-1 or TIAR is required for DT40 cell viability.  |  Le Guiner, C., et al. 2003. J Biol Chem. 278: 10465-76. PMID: 12533540
4. Cellular protein TIA-1 regulates the expression of HBV surface antigen by binding the HBV posttranscriptional regulatory element.  |  Tang, H., et al. 2008. Intervirology. 51: 203-9. PMID: 18753794
5. TIA-1 cytotoxic granule-associated RNA binding protein improves the prognostic performance of CD8 in mismatch repair-proficient colorectal cancer.  |  Zlobec, I., et al. 2010. PLoS One. 5: e14282. PMID: 21179245
6. RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions.  |  Cruz-Gallardo, I., et al. 2013. J Biol Chem. 288: 25986-25994. PMID: 23902765
7. The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation.  |  Albornoz, A., et al. 2014. J Virol. 88: 6611-22. PMID: 24696465
8. mRNA-binding protein TIA-1 reduces cytokine expression in human endometrial stromal cells and is down-regulated in ectopic endometrium.  |  Karalok, HM., et al. 2014. J Clin Endocrinol Metab. 99: E2610-9. PMID: 25140393
9. Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.  |  Motohashi, H., et al. 2017. Biochem J. 474: 1669-1687. PMID: 28298474
10. Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression.  |  Chen, B., et al. 2022. Discov Oncol. 13: 56. PMID: 35776220

The preferred TIA-1/TIAR antibody is TIA-1/TIAR Antibody (D-9): sc-48371.