TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h): sc-400153-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h) and TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TGFBR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGF beta Receptor 1/TGFBR1 Antibody (D-1): sc-518018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h) | sc-400153-NIC | 20 µg | $410.00 | |||
TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h2) | sc-400153-NIC-2 | 20 µg | $410.00 |
TGFBR1 encodes transforming growth factor beta receptor type I (ALK5), a serine/threonine kinase that pairs with TGFBR2 to transduce TGF-β ligands into intracellular signaling. Upon activation, TGFBR1 phosphorylates SMAD2/3 to drive SMAD-dependent transcriptional programs and also interfaces with non-canonical pathways including MAPK, PI3K/AKT, and Rho-like GTPase signaling, shaping cell-cycle control, differentiation, immune regulation, and extracellular matrix remodeling. Perturbation of TGFBR1 signaling is linked to altered epithelial–mesenchymal transition, fibrosis-associated transcriptional states, and dysregulated growth control observed across developmental disorders and cancer biology. As a central node in TGF-β signaling, TGFBR1 is frequently interrogated to map context-specific pathway wiring and receptor-proximal signal integration.
TGF beta Receptor 1/TGFBR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TGFBR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TGFBR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TGFBR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TGFBR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.