TFIIIC90 Double Nickase Plasmid (h): sc-411269-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIIC90 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFIIIC90 Double Nickase Plasmid (h) and TFIIIC90 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GTF3C4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIIC90 Double Nickase Plasmid (h) | sc-411269-NIC | 20 µg | $410.00 | |||
TFIIIC90 Double Nickase Plasmid (h2) | sc-411269-NIC-2 | 20 µg | $410.00 |
Human GTF3C4 encodes TFIIIC90, a core subunit of the TFIIIC complex that recognizes internal promoter elements within RNA polymerase III–transcribed genes, including tRNAs and other small noncoding RNAs. By supporting assembly of the Pol III preinitiation machinery and influencing chromatin organization at Pol III loci, TFIIIC90 contributes to small RNA homeostasis, translational capacity, and broader transcriptional network coordination. TFIIIC-dependent regulation intersects with cellular growth control, stress responses, and genome architecture through its roles at repetitive elements and barrier/insulator-like functions in certain genomic contexts. Dysregulation of Pol III transcriptional programs and TFIIIC components has been associated with proliferative phenotypes and altered RNA metabolism, making TFIIIC90 a useful target for mechanistic studies in cancer-relevant and ribostasis pathways.
TFIIIC90 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GTF3C4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GTF3C4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GTF3C4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GTF3C4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.