TERT Double Nickase Plasmid (h): sc-400316-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TERT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TERT Double Nickase Plasmid (h) and TERT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TERT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TERT Antibody (A-6): sc-393013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TERT Double Nickase Plasmid (h) | sc-400316-NIC | 20 µg | $410.00 | |||
TERT Double Nickase Plasmid (h2) | sc-400316-NIC-2 | 20 µg | $410.00 |
Human TERT encodes the catalytic reverse transcriptase subunit of telomerase, which elongates telomeric DNA repeats and counteracts progressive telomere shortening during DNA replication. Through regulation of telomere length and telomere capping, TERT supports chromosomal stability, replicative capacity, and DNA damage checkpoint signaling, intersecting with pathways such as ATM/ATR-mediated responses and senescence programs. Altered TERT activity is linked to telomere biology disorders, genome instability, and oncogenic processes, making it a central node for studying cellular immortalization, stress-induced senescence, and clonal evolution. TERT is also investigated for non-canonical roles in transcriptional regulation and mitochondrial homeostasis that can influence proliferation and stress tolerance.
TERT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TERT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TERT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TERT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TERT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.