TCR V β 7 Antibody (3H3005): sc-73129

TCR V β 7 (3H3005) is a rat monoclonal IgG2b antibody that detects the TCR V beta 7 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). TCR V β 7 (clone 3H3005) antibody is available as both non-conjugated form and multiple conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC). The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roughly 2-5% express the γ/δ heterodimer. Recognizing such a variety of antigens requires diverse specificities in the TCR repertoire. This is obtained by the somatic recombination of variable (V), diversity (D) and joining (J) gene segments in the assembly of each TCR chain. The TCR β and γ chain genes lie in distinct loci, while the genes encoding the TCR α and δ chains comprise a single locus. During T cell development, the β chain is synthesized by first joining a D segment with a J segment, then adding a V segment with the D-J gene, and later a C segment. Genetic mutations involving the T cell receptor β locus have been associated with T cell lymphomas.

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TCR V β 7 Antibody (3H3005) References:

1. Human invariant V alpha 24-J alpha Q TCR supports the development of CD1d-dependent NK1.1+ and NK1.1- T cells in transgenic mice.  |  Capone, M., et al. 2003. J Immunol. 170: 2390-8. PMID: 12594262
2. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype.  |  Okada, CY., et al. 1990. J Immunol. 144: 3473-7. PMID: 1691759
3. Differential recognition of CD1d-alpha-galactosyl ceramide by the V beta 8.2 and V beta 7 semi-invariant NKT T cell receptors.  |  Pellicci, DG., et al. 2009. Immunity. 31: 47-59. PMID: 19592275
4. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.  |  Behlke, MA., et al. 1987. J Exp Med. 165: 257-62. PMID: 2432150
5. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.  |  Staerz, UD., et al. 1985. J Immunol. 134: 3994-4000. PMID: 2580901
6. Methods for the selection and growth of antigen-specific cytolytic T lines and clones bearing a defined T cell receptor beta chain marker.  |  Ito, M., et al. 1987. J Immunol Methods. 103: 229-37. PMID: 2959725
7. A mouse monoclonal antibody specific for the V beta 5.3 chain of the human TcR recognizes a subgroup of the mouse TcR V beta 8.2 chains.  |  Bleux, C., et al. 1995. J Leukoc Biol. 57: 491. PMID: 7884322
8. Dominant V beta 8 gene usage in response to TNP: failure to use other V beta chains following removal of V beta 8+ T cells by monoclonal antibody in vivo.  |  Dieli, F., et al. 1994. Immunology. 82: 99-105. PMID: 8045598
9. T cell vaccination against autoimmune diabetes in nonobese diabetic mice.  |  Formby, B. and Shao, T. 1993. Ann Clin Lab Sci. 23: 137-47. PMID: 8096128
10. Identification of two V beta 7-specific viral superantigens.  |  Ignatowicz, L., et al. 1994. J Immunol. 152: 65-71. PMID: 8254207
11. Involvement of TCR-V beta 8.3+ cells in the cure of mice bearing a large MOPC-315 tumor by low dose melphalan.  |  Mokyr, MB., et al. 1993. J Immunol. 151: 4838-46. PMID: 8409442
12. Enhanced prevalence of T cell receptor V beta 7 gene family expression in human intestine-associated T lymphocytes.  |  Esin, S., et al. 1996. Immunol Lett. 51: 149-55. PMID: 8832283

The preferred TCR β antibody is TCR β Antibody (G-11): sc-5277.