TACC2 Double Nickase Plasmid (h): sc-405864-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TACC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TACC2 Double Nickase Plasmid (h) and TACC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TACC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TACC2 Antibody (H-8): sc-515342
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TACC2 Double Nickase Plasmid (h) | sc-405864-NIC | 20 µg | $410.00 | |||
TACC2 Double Nickase Plasmid (h2) | sc-405864-NIC-2 | 20 µg | $410.00 |
TACC2 (transforming acidic coiled-coil containing protein 2) encodes a centrosome- and microtubule-associated factor implicated in organizing the mitotic spindle and regulating microtubule dynamics during cell division. As part of TACC family–dependent processes, TACC2 contributes to centrosomal integrity, chromosome segregation, and maintenance of genomic stability through coordinated control of mitotic progression. Altered TACC2 expression or copy number has been reported in multiple tumor contexts and is studied for links to proliferative signaling, chromosomal instability, and cell-cycle dysregulation. These features make TACC2 a useful target for investigating mechanisms of mitosis, centrosome function, and microtubule-dependent trafficking in human cells.
TACC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TACC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TACC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TACC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TACC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.