Syne-1 CRISPR/Cas9 KO Plasmid (h): sc-402804
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Syne-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Syne-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Syne-1 Antibody (3G2): sc-293465
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syne-1 CRISPR/Cas9 KO Plasmid (h) | sc-402804 | 20 µg | $397.00 |
Overview
SYNE1 encodes Syne-1 (nesprin-1), a large spectrin-repeat–containing scaffold of the LINC complex that couples the nucleus to the cytoskeleton through interactions with SUN proteins, actin, and intermediate filaments. By regulating nuclear positioning, mechanotransduction, and cytoskeletal organization, Syne-1 influences processes such as cell migration, polarity, and tissue architecture. Disruption of SYNE1 has been linked to neurodevelopmental and neurodegenerative phenotypes, including autosomal recessive cerebellar ataxia, and has also been reported in cancer genomics studies where altered nuclear-cytoskeletal coupling can affect genome stability and cell behavior. These features make SYNE1 a relevant target for investigating nuclear envelope biology, force transmission, and disease-associated cellular phenotypes in human model systems.
Syne-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SYNE1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SYNE1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SYNE1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Syne-1 protein expression.
This CRISPR knockout system enables efficient generation of SYNE1-deficient cell models for investigation of Syne-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SYNE1 exon(s) critical for Syne-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SYNE1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Syne-1 CRISPR/Cas9 KO Plasmid (h) and Syne-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SYNE1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Syne-1 HDR Plasmid (h) and Syne-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SYNE1 homology arms to support homology-directed repair at defined SYNE1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.