Svs5 CRISPR/Cas9 KO Plasmid (m): sc-423227
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Svs5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Svs5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Svs5 CRISPR/Cas9 KO Plasmid (m) | sc-423227 | 20 µg | $397.00 |
Overview
Svs5 encodes seminal vesicle secretory protein 5 (Svs5), a mouse reproductive tract protein associated with male accessory gland secretions and the post-ejaculatory environment. Svs family proteins are implicated in processes such as semen coagulation, sperm protection, and modulation of sperm function through interactions with extracellular protein networks and proteolytic remodeling. Although Svs5 is less well characterized than some related seminal vesicle proteins, its expression profile makes it relevant to studies of androgen-regulated gene programs, reproductive physiology, and factors influencing male fertility phenotypes. Altered regulation of accessory gland secretory proteins can inform mechanisms underlying subfertility and reproductive tract biology in mouse models.
Svs5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Svs5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Svs5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Svs5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Svs5 protein expression.
This CRISPR knockout system enables efficient generation of Svs5-deficient cell models for investigation of Svs5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Svs5 exon(s) critical for Svs5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Svs5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Svs5 CRISPR/Cas9 KO Plasmid (m) and Svs5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Svs5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Svs5 HDR Plasmid (m) and Svs5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Svs5 homology arms to support homology-directed repair at defined Svs5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.