STOP Antibody (H-8): sc-137036

STOP Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects STOP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-STOP antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. STOP, consisting of 952 amino acids, plays a crucial role in stabilizing microtubules within the cytoplasm of mammalian cells, particularly under conditions that typically promote depolymerization, such as exposure to cold temperatures or assembly-inhibiting drugs. This stabilization is vital for maintaining cellular structure and function, especially in neurons, where STOP is predominantly located in the cell body and axon. The central region of STOP features five tandem repeats of 46 amino acids, which are essential for microtubule-binding properties, while a SH3-binding motif near the N-terminus facilitates interactions with other proteins, further underscoring STOP′s importance in cellular dynamics. STOP′s ability to confer resistance to extreme temperatures, even as low as -80° C, highlights STOP′s significance in preserving microtubule integrity and supporting neuronal health, making STOP monoclonal antibody (H-8) an invaluable tool for researchers studying microtubule dynamics and related cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

STOP Antibody (H-8) References:

1. STOP (stable-tubule-only-polypeptide) is preferentially associated with the stable domain of axonal microtubules.  |  Slaughter, T. and Black, MM. 2003. J Neurocytol. 32: 399-413. PMID: 14724383
2. Astrocytes and oligodendrocytes express different STOP protein isoforms.  |  Galiano, MR., et al. 2004. J Neurosci Res. 78: 329-37. PMID: 15389836
3. Specific association of STOP protein with microtubules in vitro and with stable microtubules in mitotic spindles of cultured cells.  |  Margolis, RL., et al. 1990. EMBO J. 9: 4095-102. PMID: 2249667
4. Monoclonal antibody to microtubule-associated STOP protein: affinity purification of neuronal STOP activity and comparison of antigen with activity in neuronal and nonneuronal cell extracts.  |  Pirollet, F., et al. 1989. Biochemistry. 28: 835-42. PMID: 2713350
5. High concentrations of STOP protein induce a microtubule super-stable state.  |  Job, D., et al. 1987. Biochem Biophys Res Commun. 148: 429-34. PMID: 3675590
6. Purification and assay of cold-stable microtubules and STOP protein.  |  Margolis, RL., et al. 1986. Methods Enzymol. 134: 160-70. PMID: 3821559
7. Some common properties between a brain protein that is modified by posttranslational arginylation and the microtubule-associated STOP protein.  |  Bongiovanni, G., et al. 1994. J Neurochem. 63: 2295-9. PMID: 7964750
8. Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts.  |  Denarier, E., et al. 1998. Proc Natl Acad Sci U S A. 95: 6055-60. PMID: 9600916
9. STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells.  |  Guillaud, L., et al. 1998. J Cell Biol. 142: 167-79. PMID: 9660871

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STOP Antibody (H-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-STOP Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STOP Antibody (H-8) Specifications

Alternate Product Name: STOP

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: STOP

Epitope Species: mouse

Product Reactivity/Detection: STOP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for STOP Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin