Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stim1 CRISPR/Cas9 KO Plasmid (h): sc-401311
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stim1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Stim1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stim1 Antibody (A-8): sc-166840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stim1 CRISPR/Cas9 KO Plasmid (h) | sc-401311 | 20 µg | $397.00 |
Overview
STIM1 encodes stromal interaction molecule 1 (Stim1), an endoplasmic reticulum Ca2+ sensor that initiates store-operated calcium entry by coupling ER Ca2+ depletion to activation of ORAI channels at ER–plasma membrane junctions. This Ca2+ influx regulates NFAT-dependent transcription, cytoskeletal remodeling, secretion, and broader calcium-dependent signaling networks controlling proliferation and stress responses. STIM1-dependent pathways are central to immune cell activation and other excitable and non-excitable cell functions, linking altered calcium homeostasis to immunological phenotypes and additional pathophysiology. In human disease biology, dysregulated STIM1 signaling is frequently studied in the context of aberrant Ca2+ entry and downstream transcriptional programs that impact cell-state decisions.
Stim1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STIM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STIM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STIM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Stim1 protein expression.
This CRISPR knockout system enables efficient generation of STIM1-deficient cell models for investigation of Stim1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STIM1 exon(s) critical for Stim1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STIM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Stim1 CRISPR/Cas9 KO Plasmid (h) and Stim1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STIM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Stim1 HDR Plasmid (h) and Stim1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STIM1 homology arms to support homology-directed repair at defined STIM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.