STC2 Double Nickase Plasmid (h): sc-405292-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- STC2 Double Nickase Plasmid (h) and STC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: STC2 Antibody (2B11): sc-293388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STC2 Double Nickase Plasmid (h) | sc-405292-NIC | 20 µg | $410.00 | |||
STC2 Double Nickase Plasmid (h2) | sc-405292-NIC-2 | 20 µg | $410.00 |
Stanniocalcin 2 (STC2) is a secreted glycoprotein hormone implicated in calcium and phosphate homeostasis and broader stress-adaptive programs in human cells. It is regulated by hypoxia and endoplasmic reticulum stress signaling and intersects with pathways controlling oxidative stress, mitochondrial metabolism, and cell survival, including HIF-1 and unfolded protein response networks. STC2 expression has been linked to altered proliferative and invasive phenotypes and is frequently studied in the context of tumor biology, where it correlates with microenvironmental stress responses. Beyond oncology, STC2 is investigated in metabolic and cardiovascular research for its roles in cellular protection, tissue remodeling, and inflammation-associated signaling.
STC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.