Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
STAU1 Double Nickase Plasmid (h): sc-402630-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STAU1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- STAU1 Double Nickase Plasmid (h) and STAU1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAU1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: STAU1 Antibody (C-4): sc-390820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STAU1 Double Nickase Plasmid (h) | sc-402630-NIC | 20 µg | $410.00 | |||
STAU1 Double Nickase Plasmid (h2) | sc-402630-NIC-2 | 20 µg | $410.00 |
STAU1 (Staufen double-stranded RNA binding protein 1) is a conserved RNA-binding protein that recognizes structured RNAs and regulates post-transcriptional gene expression through mRNA localization, stability, and translation. It is a core factor in Staufen-mediated mRNA decay (SMD), linking double-stranded RNA elements in target transcripts to turnover pathways that shape proteome output. STAU1 also participates in ribonucleoprotein granule dynamics and stress responses, influencing RNA trafficking and translational control in the cytoplasm. Dysregulated STAU1-dependent RNA metabolism has been implicated in altered neuronal and muscle homeostasis and is frequently studied in the context of neurodegeneration and RNA granule–associated disorders.
STAU1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAU1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAU1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAU1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAU1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.