Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stat6 Double Nickase Plasmid (h): sc-418159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat6 Double Nickase Plasmid (h) and Stat6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAT6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat6 Antibody (D-1): sc-374021
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat6 Double Nickase Plasmid (h) | sc-418159-NIC | 20 µg | $410.00 | |||
Stat6 Double Nickase Plasmid (h2) | sc-418159-NIC-2 | 20 µg | $410.00 |
STAT6 encodes Stat6, a latent transcription factor activated downstream of IL-4 and IL-13 receptor signaling through JAK kinases and subsequent phosphorylation-dependent dimerization and nuclear translocation. In the nucleus, Stat6 binds regulatory DNA elements to control gene programs involved in Th2 differentiation, alternative macrophage activation, epithelial remodeling, and immunoglobulin class switching. This pathway integrates cytokine cues with chromatin and transcriptional machinery to shape allergic inflammation and type 2 immune responses. Dysregulated STAT6 activity is implicated in asthma and atopic disease mechanisms and is also studied in contexts of tumor-associated immune polarization and cytokine-driven cell-state transitions.
Stat6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAT6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAT6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAT6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAT6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.