Stat5a Double Nickase Plasmid (h): sc-400150-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat5a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat5a Double Nickase Plasmid (h) and Stat5a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAT5A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat5a Antibody (C-6): sc-271542
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat5a Double Nickase Plasmid (h) | sc-400150-NIC | 20 µg | $410.00 | |||
Stat5a Double Nickase Plasmid (h2) | sc-400150-NIC-2 | 20 µg | $410.00 |
STAT5A encodes Stat5a, a cytokine-activated transcription factor that is phosphorylated downstream of JAK kinases following stimulation by receptors for interleukins, growth hormone, prolactin, and other hematopoietic signals. Activated Stat5a dimerizes, translocates to the nucleus, and regulates gene programs controlling proliferation, survival, differentiation, and immune cell homeostasis, integrating crosstalk with PI3K–AKT and MAPK signaling. STAT5A activity is frequently studied in contexts of dysregulated cytokine signaling and altered transcriptional control in hematologic malignancies and immune-mediated disease models. Perturbing STAT5A enables mechanistic dissection of lineage specification, cytokine dependence, and transcriptional network rewiring in human cells.
Stat5a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAT5A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAT5A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAT5A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAT5A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.