Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Stat3 Double Nickase Plasmid (h): sc-400027-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Stat3 Double Nickase Plasmid (h) and Stat3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STAT3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat3 Antibody (F-2): sc-8019
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat3 Double Nickase Plasmid (h) | sc-400027-NIC | 20 µg | $410.00 | |||
Stat3 Double Nickase Plasmid (h2) | sc-400027-NIC-2 | 20 µg | $410.00 |
STAT3 encodes Stat3, a latent cytoplasmic transcription factor activated downstream of cytokine and growth factor receptors, prominently via JAK kinases. Upon phosphorylation, Stat3 dimerizes and translocates to the nucleus to regulate gene programs controlling proliferation, survival, differentiation, inflammation, and immune evasion, with cross-talk to MAPK and PI3K–AKT signaling. STAT3 activity is frequently dysregulated in cancer biology, chronic inflammatory states, and immune-mediated disorders, and it is widely used as a node for studying transcriptional control of cytokine signaling. In human cells, STAT3-dependent outputs include acute-phase responses, T cell polarization, and epithelial–mesenchymal transition-associated transcriptional networks.
Stat3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STAT3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STAT3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STAT3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STAT3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.