SPARC CRISPR/Cas9 KO Plasmid (m): sc-423101
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPARC CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPARC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPARC Antibody (AON-1): sc-33645
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPARC CRISPR/Cas9 KO Plasmid (m) | sc-423101 | 20 µg | $397.00 |
Overview
Mouse Sparc encodes SPARC (secreted protein acidic and rich in cysteine), a matricellular glycoprotein that modulates extracellular matrix organization, collagen deposition, and cell–matrix interactions. SPARC influences integrin signaling, focal adhesion dynamics, and growth factor availability, shaping processes such as adhesion, migration, proliferation, and tissue remodeling. Altered SPARC expression is linked to fibrosis and aberrant stromal remodeling, and it is frequently studied in contexts of wound repair, vascular biology, and tumor microenvironment regulation. In immune and musculoskeletal systems, SPARC-dependent matrix cues can impact inflammation, osteogenesis, and mechanical properties of connective tissues.
SPARC CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sparc gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sparc together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sparc open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPARC protein expression.
This CRISPR knockout system enables efficient generation of Sparc-deficient cell models for investigation of SPARC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sparc exon(s) critical for SPARC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sparc genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPARC CRISPR/Cas9 KO Plasmid (m) and SPARC CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sparc locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPARC HDR Plasmid (m) and SPARC HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sparc homology arms to support homology-directed repair at defined Sparc target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.