Sp9 CRISPR/Cas9 KO Plasmid (m): sc-436278
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sp9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sp9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp9 CRISPR/Cas9 KO Plasmid (m) | sc-436278 | 20 µg | $397.00 |
Overview
Sp9 (Sp9 transcription factor) is a member of the Sp/KLF family of GC-box binding proteins that regulates gene expression programs controlling cell fate decisions during embryonic development. In mouse, Sp9 activity has been linked to patterning and differentiation processes in the central nervous system and limb, where it coordinates transcriptional networks that influence progenitor proliferation and tissue morphogenesis. As a nuclear DNA-binding regulator, Sp9 interfaces with broader developmental signaling contexts, including pathways that converge on enhancer and promoter architecture to tune lineage-specific transcription. Dysregulated developmental transcription factor function is frequently associated with congenital phenotypes and neurodevelopmental defects, making Sp9 a useful target for mechanistic studies of gene regulatory circuitry.
Sp9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sp9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sp9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sp9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sp9 protein expression.
This CRISPR knockout system enables efficient generation of Sp9-deficient cell models for investigation of Sp9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sp9 exon(s) critical for Sp9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sp9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sp9 CRISPR/Cas9 KO Plasmid (m) and Sp9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sp9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sp9 HDR Plasmid (m) and Sp9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sp9 homology arms to support homology-directed repair at defined Sp9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.