Sox2 Lentiviral Activation Particles (h): sc-400050-LAC

Human SOX2 encodes Sox2, an HMG-box transcription factor that binds regulatory DNA to control gene expression programs governing pluripotency, embryonic lineage specification, and neural progenitor maintenance. Sox2 cooperates with core stem cell factors to modulate chromatin accessibility and transcriptional networks that influence self-renewal, differentiation trajectories, and cell fate stability. Dysregulated SOX2 expression is associated with aberrant developmental patterning and has been widely studied in contexts such as cancer stem-like states, lineage plasticity, and resistance to differentiation cues. As a central node in transcriptional and epigenetic control, SOX2 is frequently interrogated in pathways linked to WNT, NOTCH, and growth factor signaling cross-talk that shape stemness and neural development.

Sox2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SOX2 upregulation across a broader range of human cell types.

Sox2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SOX2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Sox2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SOX2 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.