SOAT2 Antibody (ACAT-2): sc-69837

SOAT2 Antibody (ACAT-2) is a mouse monoclonal IgG1 antibody that detects SOAT2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-SOAT2 antibody (ACAT-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SOAT2, also known as acyl-CoA:cholesterol acyltransferase-2, plays a crucial role in lipid metabolism by catalyzing the esterification of cholesterol, which is essential for the assembly of lipoproteins in mammalian cells. This process is particularly important as SOAT2 facilitates the transport of cholesterol and triglycerides in the bloodstream, thereby influencing cellular cholesterol homeostasis and preventing the accumulation of free cholesterol that can lead to cellular toxicity. SOAT2 is predominantly localized in the endoplasmic reticulum of human intestinal cells, where SOAT2 is involved in the synthesis of cholesterol esters, a key step in the formation of chylomicrons and very low-density lipoproteins (VLDL). Deficiencies in SOAT2 can lead to severe metabolic disorders, including mental retardation and hypotonia, highlighting SOAT2′s significance in both lipid metabolism and overall health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SOAT2 Antibody (ACAT-2) References:

1. Human acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) gene organization and evidence that the 4.3-kilobase ACAT-1 mRNA is produced from two different chromosomes.  |  Li, BL., et al. 1999. J Biol Chem. 274: 11060-71. PMID: 10196189
2. Human acyl-CoA:cholesterol acyltransferase-1 in the endoplasmic reticulum contains seven transmembrane domains.  |  Lin, S., et al. 1999. J Biol Chem. 274: 23276-85. PMID: 10438503
3. Human acyl-CoA:cholesterol acyltransferase-1 is a homotetrameric enzyme in intact cells and in vitro.  |  Yu, C., et al. 1999. J Biol Chem. 274: 36139-45. PMID: 10593897
4. Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine.  |  Chang, CC., et al. 2000. J Biol Chem. 275: 28083-92. PMID: 10846185
5. Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D(3) or 9-cis-retinoic acid in undifferentiated THP-1 cells.  |  Maung, K., et al. 2001. J Lipid Res. 42: 181-7. PMID: 11181746
6. Synergistic transcriptional activation of human Acyl-coenzyme A: cholesterol acyltransterase-1 gene by interferon-gamma and all-trans-retinoic acid THP-1 cells.  |  Yang, JB., et al. 2001. J Biol Chem. 276: 20989-98. PMID: 11399774
7. Direct effect of an acyl-CoA:cholesterol acyltransferase inhibitor, F-1394, on atherosclerosis in apolipoprotein E and low density lipoprotein receptor double knockout mice.  |  Chiwata, T., et al. 2001. Br J Pharmacol. 133: 1005-12. PMID: 11487509
8. Acyl-coenzyme A: cholesterol acyltransferase modulates the generation of the amyloid beta-peptide.  |  Puglielli, L., et al. 2001. Nat Cell Biol. 3: 905-12. PMID: 11584272
9. Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner.  |  Chang, CC., et al. 1998. J Biol Chem. 273: 35132-41. PMID: 9857049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SOAT2 Antibody (ACAT-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SOAT2 Antibody (ACAT-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SOAT2 Antibody (ACAT-2) Specifications

Alternate Product Name: SOAT2

Product Clone Name: ACAT-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 314-388

Epitope Target: SOAT2

Epitope Species: human

Product Reactivity/Detection: SOAT2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SOAT2 Antibody (ACAT-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin