Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNX3 CRISPR/Cas9 KO Plasmid (h): sc-404028
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNX3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX3 Antibody (G-7): sc-376667
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX3 CRISPR/Cas9 KO Plasmid (h) | sc-404028 | 20 µg | $397.00 |
Overview
Human SNX3 encodes sorting nexin 3, a PX domain–containing endosomal protein that binds phosphatidylinositol 3-phosphate and helps organize membrane trafficking on early endosomes. SNX3 functions as a key accessory of the retromer pathway, supporting endosome-to-Golgi and endosome-to-plasma membrane recycling of specific transmembrane cargos and contributing to receptor sorting, nutrient transporter homeostasis, and signal attenuation. Through its role in endosomal sorting and cargo retrieval, SNX3 influences processes linked to Wnt signaling, membrane receptor turnover, and cellular polarity. Dysregulated endosomal trafficking and retromer-associated cargo mis-sorting are implicated in neurodegenerative and cancer-associated phenotypes, making SNX3 a useful node for mechanistic studies of trafficking-dependent disease biology.
SNX3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SNX3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SNX3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SNX3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNX3 protein expression.
This CRISPR knockout system enables efficient generation of SNX3-deficient cell models for investigation of SNX3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SNX3 exon(s) critical for SNX3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SNX3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNX3 CRISPR/Cas9 KO Plasmid (h) and SNX3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SNX3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNX3 HDR Plasmid (h) and SNX3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SNX3 homology arms to support homology-directed repair at defined SNX3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.