Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNAP 25 Double Nickase Plasmid (m): sc-423048-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNAP 25 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SNAP 25 Double Nickase Plasmid (m) and SNAP 25 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Snap25. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNAP 25 Antibody (SP12): sc-20038
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAP 25 Double Nickase Plasmid (m) | sc-423048-NIC | 20 µg | $410.00 |
Snap25 encodes synaptosomal-associated protein 25 (SNAP25), a core component of the neuronal SNARE complex that drives synaptic vesicle docking and calcium-triggered membrane fusion during neurotransmitter release. By partnering with syntaxin and VAMP/synaptobrevin, SNAP25 regulates presynaptic exocytosis, short-term synaptic plasticity, and activity-dependent vesicle recycling in excitatory and inhibitory circuits. SNAP25-dependent signaling intersects with pathways controlling neuronal excitability and network development, making Snap25 a widely used locus for probing synaptic transmission mechanisms in mouse models. Altered SNAP25 expression or SNARE function has been linked to neurodevelopmental and neuropsychiatric phenotypes in genetic and functional studies, supporting its relevance for research on circuit dysfunction.
SNAP 25 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Snap25 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Snap25. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Snap25 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Snap25-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.