SMC1α Antibody (E-8): sc-166734
- SMC1α Antibody (E-8) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 545-556 within an internal region of SMC1α of human origin
- recommended for detection of SMC1α of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SMC1α Antibody (E-8). This work is in progress.
SMC1α Antibody (E-8) is a mouse monoclonal IgM antibody that detects SMC1α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SMC1α, also known as structural maintenance of chromosomes protein 1A, is a crucial 1,233 amino acid nuclear protein that plays a vital role in maintaining chromosome cohesion during the cell cycle. SMC1α is a key component of the cohesion complex, which is essential for the proper alignment and separation of sister chromatids following DNA replication. SMC1α′s importance extends beyond its structural role; SMC1α interacts with BRCA1 and is phosphorylated by ATM, highlighting SMC1α′s involvement in DNA repair mechanisms. Mutations in the gene encoding SMC1α have been linked to Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by facial dysmorphia, upper limb malformations, and cognitive impairments. Anti-SMC1α antibody (E-8) provides a valuable tool for researchers studying chromosome dynamics and molecular pathways associated with genomic stability and developmental disorders.
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SMC1α Antibody (E-8) References:
- Novel meiosis-specific isoform of mammalian SMC1. | Revenkova, E., et al. 2001. Mol Cell Biol. 21: 6984-98. PMID: 11564881
- DNA interaction and dimerization of eukaryotic SMC hinge domains. | Chiu, A., et al. 2004. J Biol Chem. 279: 26233-42. PMID: 15087462
- Proteomic analysis of SRm160-containing complexes reveals a conserved association with cohesin. | McCracken, S., et al. 2005. J Biol Chem. 280: 42227-36. PMID: 16159877
- Relationship between incomplete synapsis and chiasma localization. | Viera, A., et al. 2009. Chromosoma. 118: 377-89. PMID: 19238420
- RAD21L, a novel cohesin subunit implicated in linking homologous chromosomes in mammalian meiosis. | Lee, J. and Hirano, T. 2011. J Cell Biol. 192: 263-76. PMID: 21242291
- Identification and molecular characterization of the mammalian α-kleisin RAD21L. | Gutiérrez-Caballero, C., et al. 2011. Cell Cycle. 10: 1477-87. PMID: 21527826
- Meiotic cohesin SMC1β provides prophase I centromeric cohesion and is required for multiple synapsis-associated functions. | Biswas, U., et al. 2013. PLoS Genet. 9: e1003985. PMID: 24385917
- Genetic Interactions Between the Meiosis-Specific Cohesin Components, STAG3, REC8, and RAD21L. | Ward, A., et al. 2016. G3 (Bethesda). 6: 1713-24. PMID: 27172213
- Distinct Roles of Meiosis-Specific Cohesin Complexes in Mammalian Spermatogenesis. | Biswas, U., et al. 2016. PLoS Genet. 12: e1006389. PMID: 27792785
- SMC1α Substitutes for Many Meiotic Functions of SMC1β but Cannot Protect Telomeres from Damage. | Biswas, U., et al. 2018. Curr Biol. 28: 249-261.e4. PMID: 29337080
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMC1α Antibody (E-8) | sc-166734 | 200 µg/ml | $322.00 | |||
SMC1α (E-8) Neutralizing Peptide | sc-166734 P | 100 µg/0.5 ml | $69.00 |