SLC26A3 Double Nickase Plasmid (h): sc-403891-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC26A3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC26A3 Double Nickase Plasmid (h) and SLC26A3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC26A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLC26A3 Antibody (H-8): sc-376187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC26A3 Double Nickase Plasmid (h) | sc-403891-NIC | 20 µg | $410.00 | |||
SLC26A3 Double Nickase Plasmid (h2) | sc-403891-NIC-2 | 20 µg | $410.00 |
Human SLC26A3 encodes a membrane anion exchanger (DRA) that mediates electroneutral chloride/bicarbonate exchange and contributes to intestinal epithelial electrolyte absorption and luminal pH homeostasis. Through regulation of transepithelial ion flux, SLC26A3 interfaces with processes governing fluid balance, mucus layer stability, and epithelial barrier function in the gastrointestinal tract. Altered SLC26A3 activity is associated with congenital chloride diarrhea and has been implicated in broader mechanisms of epithelial dysregulation, including inflammatory and neoplastic contexts in the colon. As a transporter influencing local ionic microenvironments, SLC26A3 is relevant to studies of enterocyte differentiation, transporter networks, and ion-coupled signaling.
SLC26A3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC26A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC26A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC26A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC26A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.