Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SF2/ASF Double Nickase Plasmid (h): sc-400737-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SF2/ASF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SF2/ASF Double Nickase Plasmid (h) and SF2/ASF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SRSF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SF2/ASF Antibody (96): sc-33652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SF2/ASF Double Nickase Plasmid (h) | sc-400737-NIC | 20 µg | $410.00 | |||
SF2/ASF Double Nickase Plasmid (h2) | sc-400737-NIC-2 | 20 µg | $410.00 |
Human SRSF1 encodes the splicing factor SF2/ASF, an SR-rich RNA-binding protein that couples pre-mRNA splicing to mRNA export, stability, and translation. By recognizing exonic splicing enhancers and coordinating spliceosome assembly, SF2/ASF shapes alternative splicing programs that influence cell-cycle progression, apoptosis, and stress-responsive gene expression. SRSF1 activity is integrated with phosphorylation-dependent signaling and RNA processing networks, including regulation of transcript isoforms that modulate MAPK/PI3K-associated outputs. Dysregulated SRSF1 expression or splicing control has been linked to aberrant isoform usage and oncogenic phenotypes in multiple tumor contexts, making it a key node for studying RNA-processing–driven disease mechanisms.
SF2/ASF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SRSF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SRSF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SRSF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SRSF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.