Selenoprotein K CRISPR/Cas9 KO Plasmid (h): sc-407002
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Selenoprotein K CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Selenoprotein K genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Selenoprotein K CRISPR/Cas9 KO Plasmid (h) | sc-407002 | 20 µg | $397.00 |
Overview
SELENOK encodes selenoprotein K, a small endoplasmic reticulum membrane protein that incorporates selenocysteine and supports redox homeostasis in immune and secretory cells. Selenoprotein K participates in ER-associated quality control and is linked to calcium flux and protein palmitoylation through interaction with the DHHC6 palmitoyltransferase complex, influencing receptor signaling and membrane organization. It contributes to efficient immune cell activation, oxidative stress responses, and proteostasis under inflammatory or metabolic stress. Altered SELENOK activity has been associated with dysregulated inflammatory signaling and phenotypes relevant to immune-mediated and neurodegenerative disease biology.
Selenoprotein K CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SELENOK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SELENOK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SELENOK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Selenoprotein K protein expression.
This CRISPR knockout system enables efficient generation of SELENOK-deficient cell models for investigation of Selenoprotein K signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SELENOK exon(s) critical for Selenoprotein K function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SELENOK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Selenoprotein K CRISPR/Cas9 KO Plasmid (h) and Selenoprotein K CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SELENOK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Selenoprotein K HDR Plasmid (h) and Selenoprotein K HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SELENOK homology arms to support homology-directed repair at defined SELENOK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.