Sec6 Antibody (H-5): sc-374054
- Sec6 Antibody (H-5) is a mouse monoclonal IgG1 κ Sec6 antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 3-27 at the N-terminus of Sec6 of human origin
- Sec6 Antibody (H-5) is recommended for detection of Sec6 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and avian
- Anti-Sec6 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Sec6 Antibody (H-5). This work is in progress.
Sec6 Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sec6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sec6 (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Sec6 protein is a crucial component of the mammalian exocyst complex, which consists of eight protein subunits: Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. This complex plays a vital role in targeting exocytic vesicles to specific docking sites on the plasma membrane, thereby regulating polarized secretion in a GTP-dependent manner. Proper functioning of the exocyst complex is essential for various cellular processes, including cell signaling, growth, and tissue development, making Sec6 a significant protein in the study of cellular dynamics. Additionally, human Sec6 is located on chromosome 5p15.33, highlighting its genetic importance in relation to various physiological and pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sec6 Antibody (H-5) References:
- The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene. | Brymora, A., et al. 2001. J Biol Chem. 276: 29792-7. PMID: 11406615
- Sec6 is localized to the plasma membrane of mature synaptic terminals and is transported with secretogranin II-containing vesicles. | Vik-Mo, EO., et al. 2003. Neuroscience. 119: 73-85. PMID: 12763070
- Ultracentrifugation-based approaches to study regulation of Sec6/8 (exocyst) complex function during development of epithelial cell polarity. | Yeaman, C. 2003. Methods. 30: 198-206. PMID: 12798134
- Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells. | Yeaman, C., et al. 2004. J Cell Sci. 117: 559-70. PMID: 14709721
- The exocyst complex in polarized exocytosis. | Hsu, SC., et al. 2004. Int Rev Cytol. 233: 243-65. PMID: 15037366
- Sec6 mutations and the Drosophila exocyst complex. | Murthy, M., et al. 2005. J Cell Sci. 118: 1139-50. PMID: 15728258
- Dimerization of the exocyst protein Sec6p and its interaction with the t-SNARE Sec9p. | Sivaram, MV., et al. 2005. Biochemistry. 44: 6302-11. PMID: 15835919
- Compartmentalization of the exocyst complex in lipid rafts controls Glut4 vesicle tethering. | Inoue, M., et al. 2006. Mol Biol Cell. 17: 2303-11. PMID: 16525015
- The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. | Sivaram, MV., et al. 2006. Nat Struct Mol Biol. 13: 555-6. PMID: 16699513
- Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. | Beronja, S., et al. 2005. J Cell Biol. 169: 635-46. PMID: 15897260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec6 Antibody (H-5) | sc-374054 | 200 µg/ml | $322.00 | |||
Sec6 Antibody (H-5) AC | sc-374054 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Sec6 Antibody (H-5) HRP | sc-374054 HRP | 200 µg/ml | $322.00 | |||
Sec6 Antibody (H-5) FITC | sc-374054 FITC | 200 µg/ml | $336.00 | |||
Sec6 Antibody (H-5) PE | sc-374054 PE | 200 µg/ml | $349.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 488 | sc-374054 AF488 | 200 µg/ml | $364.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 546 | sc-374054 AF546 | 200 µg/ml | $364.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 594 | sc-374054 AF594 | 200 µg/ml | $364.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 647 | sc-374054 AF647 | 200 µg/ml | $364.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 680 | sc-374054 AF680 | 200 µg/ml | $364.00 | |||
Sec6 Antibody (H-5) Alexa Fluor® 790 | sc-374054 AF790 | 200 µg/ml | $364.00 | |||
Sec6 (H-5) Neutralizing Peptide | sc-374054 P | 100 µg/0.5 ml | $69.00 |