Scratch1 CRISPR/Cas9 KO Plasmid (h): sc-410173
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Scratch1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Scratch1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Scratch1 CRISPR/Cas9 KO Plasmid (h) | sc-410173 | 20 µg | $397.00 |
Overview
SCRT1 encodes Scratch1, a zinc finger transcription factor that acts predominantly as a transcriptional repressor in neural lineages, helping coordinate neuronal differentiation, migration, and maturation programs. Scratch1 functions within gene regulatory networks that intersect with proneural bHLH factors and EMT-related transcriptional control, shaping cell state transitions and cytoskeletal dynamics during development. By modulating lineage-specific gene expression and repression of non-neuronal programs, SCRT1 contributes to maintenance of neuronal identity and context-dependent control of proliferation. Dysregulated SCRT1-associated transcriptional programs have been investigated in neurodevelopmental phenotypes and in cancer biology where developmental regulators are frequently repurposed to influence differentiation state and invasiveness.
Scratch1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SCRT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SCRT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SCRT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Scratch1 protein expression.
This CRISPR knockout system enables efficient generation of SCRT1-deficient cell models for investigation of Scratch1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SCRT1 exon(s) critical for Scratch1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SCRT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Scratch1 CRISPR/Cas9 KO Plasmid (h) and Scratch1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SCRT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Scratch1 HDR Plasmid (h) and Scratch1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SCRT1 homology arms to support homology-directed repair at defined SCRT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.