Ron β Double Nickase Plasmid (h): sc-400489-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ron β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ron β Double Nickase Plasmid (h) and Ron β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MST1R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ron α Antibody (C-5): sc-393523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ron β Double Nickase Plasmid (h) | sc-400489-NIC | 20 µg | $410.00 | |||
Ron β Double Nickase Plasmid (h2) | sc-400489-NIC-2 | 20 µg | $410.00 |
MST1R encodes the receptor tyrosine kinase RON (also known as macrophage-stimulating 1 receptor), a member of the MET family that transduces signals from macrophage-stimulating protein (MSP). Upon activation, RON engages downstream pathways such as PI3K–AKT, RAS–MAPK, and STAT signaling to regulate epithelial cell survival, migration, and wound repair–associated programs, and it can modulate macrophage polarization and inflammatory responses. Altered MST1R/RON signaling has been linked to dysregulated cell motility and invasion phenotypes and to changes in tumor–microenvironment crosstalk across multiple cancer contexts. The Ron beta isoform provides a biologically relevant variant for dissecting isoform-specific signaling outputs and receptor crosstalk within MET-family networks.
Ron β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MST1R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MST1R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MST1R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MST1R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.