RNase HII-A Antibody (S-14LJ-17): sc-101112

RNase HII-A Antibody (S-14LJ-17) is a mouse monoclonal IgG1 antibody that detects RNase HII-A in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). RNase HII-A, also known as RNASEH2A, is a crucial 299 amino acid catalytic subunit of the RNase HII complex, which plays a vital role in DNA replication by degrading RNA found in RNA:DNA duplexes. RNase HII-A is primarily localized in the nucleus, where RNase HII-A is responsible for removing Okazaki fragment RNA primers from the lagging strand, ensuring DNA integrity and continuity during replication. RNase HII-A′s endonucleolytic activity cleaves RNA to a 5′-phosphomonoester, a process essential for maintaining genomic stability. RNase HII-A requires magnesium or manganese as cofactors for enzymatic function, highlighting the importance of metal ions in activity. Mutations in the gene encoding RNase HII-A can lead to Aicardi-Goutieres syndrome type 4 (AGS4), a severe autosomal recessive encephalopathy characterized by significant neurological impairments and early mortality, underscoring RNase HII-A′s critical nature in human health. Anti-RNase HII-A antibody (S-14LJ-17) is an invaluable tool for researchers studying DNA replication mechanisms and related disorders′ pathophysiology.

The preferred RNase HII-A antibody is RNase HII-A Antibody (G-10): sc-515475.