Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RFC4 CRISPR/Cas9 KO Plasmid (h): sc-403956
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RFC4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RFC4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RFC4 Antibody (C-9): sc-28301
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RFC4 CRISPR/Cas9 KO Plasmid (h) | sc-403956 | 20 µg | $397.00 |
Overview
RFC4 (replication factor C subunit 4) is a core component of the RFC clamp loader complex that loads PCNA onto primed DNA, enabling processive DNA synthesis during replication and coordinating DNA repair. Through its ATPase-driven interactions with PCNA and DNA polymerases, RFC4 supports S-phase progression, replication fork stability, and checkpoint-associated responses to replication stress. Disruption of RFC4 perturbs DNA replication dynamics and can increase genome instability, linking RFC complex function to pathways commonly altered in proliferative disorders. As part of the broader DNA replication and repair network, RFC4 is frequently studied in the context of cell-cycle regulation and stress responses.
RFC4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RFC4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RFC4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RFC4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RFC4 protein expression.
This CRISPR knockout system enables efficient generation of RFC4-deficient cell models for investigation of RFC4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RFC4 exon(s) critical for RFC4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RFC4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RFC4 CRISPR/Cas9 KO Plasmid (h) and RFC4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RFC4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RFC4 HDR Plasmid (h) and RFC4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RFC4 homology arms to support homology-directed repair at defined RFC4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.