RBM15 Double Nickase Plasmid (h): sc-417289-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RBM15 Double Nickase Plasmid (h) and RBM15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RBM15. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM15 Double Nickase Plasmid (h) | sc-417289-NIC | 20 µg | $410.00 | |||
RBM15 Double Nickase Plasmid (h2) | sc-417289-NIC-2 | 20 µg | $410.00 |
RBM15 (RNA binding motif protein 15) is a nuclear RNA-binding factor that regulates post-transcriptional gene expression through control of alternative splicing, RNA stability, and mRNA export. It functions in ribonucleoprotein complexes and has been linked to epigenetic and RNA modification-associated processes, including modulation of m6A-dependent RNA metabolism via interactions with core writer components. RBM15 contributes to lineage specification and hematopoietic programs, and dysregulation of RBM15-associated RNA processing has been implicated in oncogenic transcriptional networks, including chromosomal rearrangements involving RBM15 in acute leukemias. These roles make RBM15 a useful target for dissecting coupling between RNA processing and cell-state transitions in human model systems.
RBM15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RBM15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RBM15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RBM15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RBM15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.