Anti-Rad GTPase Anticuerpo B-10 es un monoclonal de ratón IgG2a (kappa light chain) Rad GTPase Anticuerpo proporcionado como 200 µg/ml
producido contra los amino ácidos 1-143 localizados en el N-terminus de origen Rad GTPase of human
Rad GTPase Anticuerpo (B-10) es recomendado para detectar Rad GTPase de human origen, mediante WB, IP, IF y ELISA
Rad GTPase Anticuerpo (B-10) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de Rad GTPase (B-10): sc-373988.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Rad GTPase Antibody (B-10) for WB applications. This reagent is now offered in a bundle with Rad GTPase Antibody (B-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Rad GTPase Antibody (B-10) is a high quality monoclonal Rad GTPase antibody (also designated Rad GTPase antibody) suitable for the detection of the Rad GTPase protein of human origin. Rad GTPase Antibody (B-10) is available as both the non-conjugated anti-Rad GTPase antibody form, as well as multiple conjugated forms of anti-Rad GTPase antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Ras-encoded family of proteins bind GDP and GTP with high affinity. They possess a low level of intrinsic GTPase activity that increases more than 100-fold when interacting with cytosolic GTPase activating protein (GAP). Ras family members include H-Ras, K-Ras, N-Ras, M-Ras, R-Ras, E-Ras, Rheb, TC 21, RASL11B, and Rad (Ras associated with diabetes) GTPase. Rad GTPase is a GTP-binding protein that is similar to Ras but has unique features. Unlike other small GTPases, Rad GTPase lacks typical prenylation motifs at its C terminus. The Rad GTPase enzyme binds calmodulin, inhibits vascular lesion formation, has low intrinsic GTPase activity and cannot be stimulated by any known GAP molecules. Rad GTPase is expressed in skeletal muscle, cardiac muscle and lung tissues and is overexpressed in the skeletal muscle tissue of individuals with type II diabetes. It is also expressed to a lesser extent in the placenta, adipose tissue and kidney.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with Rad GTPase (B-10): sc-373988 monoclonal antibody for Western blot?
Preguntado por: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
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