Pumilio 1 CRISPR/Cas9 KO Plasmid (h): sc-406309
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pumilio 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pumilio 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pumilio 1 CRISPR/Cas9 KO Plasmid (h) | sc-406309 | 20 µg | $397.00 |
Overview
PUM1 encodes Pumilio 1, a conserved RNA-binding protein that recognizes Pumilio response elements in 3′ UTRs to regulate mRNA stability, localization, and translation. By assembling post-transcriptional regulatory complexes, PUM1 shapes gene expression programs controlling cell cycle progression, differentiation, and stress-responsive remodeling of the transcriptome. Pumilio 1 participates in RNA metabolism pathways including translational repression, mRNA decay, and coordination with microRNA-mediated regulation. Dysregulated PUM1 activity has been linked to altered proteostasis and genome maintenance programs, and has been studied in the context of neurodevelopmental and neurodegenerative phenotypes as well as cancer-associated RNA regulatory networks.
Pumilio 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PUM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PUM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PUM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pumilio 1 protein expression.
This CRISPR knockout system enables efficient generation of PUM1-deficient cell models for investigation of Pumilio 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PUM1 exon(s) critical for Pumilio 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PUM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pumilio 1 CRISPR/Cas9 KO Plasmid (h) and Pumilio 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PUM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pumilio 1 HDR Plasmid (h) and Pumilio 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PUM1 homology arms to support homology-directed repair at defined PUM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.