PSGL-1 Antibody (C-9): sc-398402

PSGL-1 Antibody (C-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects PSGL-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PSGL-1 antibody (C-9) is available as a non-conjugated format. PSGL-1, also known as P-Selectin glycoprotein ligand or CD162, plays a crucial role in immune response by mediating leukocyte adhesion to endothelial cells during inflammation. This interaction is vital for recruiting immune cells to sites of injury or infection, highlighting PSGL-1′s importance in maintaining immune homeostasis and facilitating effective immune responses. Structurally, PSGL-1 exists as a disulfide-linked homodimer and is characterized as a type 1 membrane protein localized on leukocyte microvilli tips. PSGL-1′s extracellular domain is rich in serines, threonines, and prolines, featuring decameric repeats that contribute to adhesive properties. PSGL-1 must undergo specific post-translational modifications, including sialylation and fucosylation, to effectively bind to selectins such as E-Selectin and P-Selectin, underscoring functional interaction complexity in immune system.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PSGL-1 Antibody (C-9) References:

1. Engagement of PSGL-1 upregulates CSF-1 transcription via a mechanism that may involve Syk.  |  Ba, XQ., et al. 2005. Cell Immunol. 237: 1-6. PMID: 16289055
2. PSGL-1 is dispensible for the development of active experimental autoimmune encephalomyelitis in SJL/J mice.  |  Bill, R., et al. 2011. J Neuroimmunol. 232: 207-8. PMID: 21051092
3. PSGL-1/selectin and ICAM-1/CD18 interactions are involved in macrophage-induced drug resistance in myeloma.  |  Zheng, Y., et al. 2013. Leukemia. 27: 702-10. PMID: 22996336
4. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.  |  Krishnamurthy, VR., et al. 2015. Nat Commun. 6: 6387. PMID: 25824568
5. Base-modified UDP-sugars reduce cell surface levels of P-selectin glycoprotein 1 (PSGL-1) on IL-1β-stimulated human monocytes.  |  Kanabar, V., et al. 2016. Glycobiology. 26: 1059-1071. PMID: 27233805
6. PSGL-1 Is an Immune Checkpoint Regulator that Promotes T Cell Exhaustion.  |  Tinoco, R., et al. 2016. Immunity. 44: 1470. PMID: 27332735
7. Deficiency of Psgl-1 accelerates bleomycin (BLM)-induced lung fibrosis and inflammation in mice through activating PI3K/AKT.  |  Han, X. and Wang, SM. 2017. Biochem Biophys Res Commun. 491: 558-565. PMID: 28263743
8. The development of salt-sensitive hypertension regulated by PSGL-1 gene in mice.  |  Yang, Y., et al. 2018. Cell Biosci. 8: 20. PMID: 29541444
9. Deregulated PSGL-1 Expression in B Cells and Dendritic Cells May Be Implicated in Human Systemic Sclerosis Development.  |  Silván, J., et al. 2018. J Invest Dermatol. 138: 2123-2132. PMID: 29689251
10. Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding.  |  Murakami, T., et al. 2020. Proc Natl Acad Sci U S A. 117: 8055-8063. PMID: 32193343

The preferred PSGL-1 antibody is PSGL-1 Antibody (KPL1): sc-13535.