Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Prohibitin Double Nickase Plasmid (m): sc-422218-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Prohibitin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Prohibitin Double Nickase Plasmid (m) and Prohibitin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Phb. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Prohibitin Antibody (E-5): sc-377037
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prohibitin Double Nickase Plasmid (m) | sc-422218-NIC | 20 µg | $410.00 | |||
Prohibitin Double Nickase Plasmid (m2) | sc-422218-NIC-2 | 20 µg | $410.00 |
Mouse Phb encodes prohibitin, an evolutionarily conserved scaffold protein enriched in the inner mitochondrial membrane where it forms high-molecular-weight complexes with PHB2 to stabilize respiratory chain components and preserve cristae architecture. Prohibitin influences mitochondrial proteostasis, membrane potential, and mitophagy, integrating with pathways that govern oxidative phosphorylation, reactive oxygen species homeostasis, and cell-cycle control. Through these roles, PHB impacts metabolic adaptation and stress responses that are frequently perturbed in neurodegeneration, cardiometabolic dysfunction, and cancer biology. Phb is therefore a widely used node for studying mitochondrial quality control and signaling crosstalk between organelle function and nuclear transcriptional programs.
Prohibitin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Phb locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Phb. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Phb function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Phb-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.