Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Profilin-2 CRISPR/Cas9 KO Plasmid (h): sc-403456
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Profilin-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Profilin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Profilin-2 Antibody (4K-6): sc-100955
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Profilin-2 CRISPR/Cas9 KO Plasmid (h) | sc-403456 | 20 µg | $397.00 |
Overview
PFN2 encodes profilin-2, an actin-monomer binding protein that regulates actin polymerization dynamics and cytoskeletal remodeling. Profilin-2 modulates filament assembly in response to phosphoinositide signaling and proline-rich ligands, coordinating processes such as neurite outgrowth, synaptic vesicle trafficking, and activity-dependent spine plasticity. Through its role in actin network organization, PFN2 contributes to pathways governing cell shape changes, membrane dynamics, and migration-related behaviors. Altered profilin-2 function and downstream cytoskeletal control have been investigated in the context of neuronal connectivity and neurodevelopmental or neurodegenerative phenotypes where actin homeostasis is disrupted.
Profilin-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PFN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PFN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PFN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Profilin-2 protein expression.
This CRISPR knockout system enables efficient generation of PFN2-deficient cell models for investigation of Profilin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PFN2 exon(s) critical for Profilin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PFN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Profilin-2 CRISPR/Cas9 KO Plasmid (h) and Profilin-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PFN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Profilin-2 HDR Plasmid (h) and Profilin-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PFN2 homology arms to support homology-directed repair at defined PFN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.