Prickle2 Double Nickase Plasmid (m): sc-433985-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Prickle2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Prickle2 Double Nickase Plasmid (m) and Prickle2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Prickle2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prickle2 Double Nickase Plasmid (m) | sc-433985-NIC | 20 µg | $410.00 |
Mouse Prickle2 encodes a core component of the planar cell polarity (PCP) branch of Wnt signaling and functions as a scaffold that helps organize asymmetric protein complexes at the cell cortex. PRICKLE2 contributes to polarized cell behaviors, including neurite outgrowth, synapse development, and coordinated cytoskeletal dynamics through interactions with Dishevelled and related PCP factors. In the nervous system, Prickle2 supports neuronal connectivity and circuit maturation, processes that intersect with calcium signaling, actin remodeling, and vesicle trafficking. Genetic and functional studies link PRICKLE2 perturbation to neurodevelopmental and neuropsychiatric phenotypes, making it relevant for mechanistic research in brain development and synaptic biology.
Prickle2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Prickle2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Prickle2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Prickle2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Prickle2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.