POU2F3 Double Nickase Plasmid (h): sc-417495-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POU2F3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- POU2F3 Double Nickase Plasmid (h) and POU2F3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting POU2F3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POU2F3 Antibody (6D1): sc-293402
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POU2F3 Double Nickase Plasmid (h) | sc-417495-NIC | 20 µg | $410.00 |
POU2F3 encodes a POU domain transcription factor that directs lineage-specific gene expression programs in epithelial cells, with prominent roles in differentiation and maintenance of tuft/chemosensory cell identity. By binding octamer motifs and coordinating transcriptional networks, POU2F3 influences chromatin state and downstream pathways controlling cell fate decisions, barrier-associated functions, and stress-responsive signaling. Dysregulated POU2F3 expression has been linked to altered epithelial differentiation states and is frequently studied in the context of lineage plasticity and transcriptional reprogramming in cancer biology. As a master regulator, it serves as a useful node for dissecting how transcription factors shape cell identity and context-dependent signaling outputs.
POU2F3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the POU2F3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within POU2F3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt POU2F3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of POU2F3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.