Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLC β2 CRISPR/Cas9 KO Plasmid (h): sc-400984
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLC β2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLC β2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLC β2 Antibody (B-2): sc-515912
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLC β2 CRISPR/Cas9 KO Plasmid (h) | sc-400984 | 20 µg | $397.00 |
Overview
PLCB2 encodes phospholipase Cβ2 (PLCβ2), a membrane-associated effector downstream of G protein–coupled receptors that hydrolyzes PIP2 to generate IP3 and DAG, driving intracellular Ca2+ mobilization and PKC activation. This signaling axis regulates chemotaxis, degranulation, secretion, and cytoskeletal remodeling, particularly in hematopoietic lineages where PLCβ2 contributes to immune cell activation and inflammatory responses. PLCβ2 integrates into GPCR–Gαq/11 pathways and coordinates calcium-dependent transcriptional programs and vesicle trafficking. Dysregulated PLCβ2-linked signaling has been associated with aberrant leukocyte function and proliferative phenotypes in hematologic disease models, supporting its study in immunity and cancer-associated signaling networks.
PLC β2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLCB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLCB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLCB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLC β2 protein expression.
This CRISPR knockout system enables efficient generation of PLCB2-deficient cell models for investigation of PLC β2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLCB2 exon(s) critical for PLC β2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLCB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLC β2 CRISPR/Cas9 KO Plasmid (h) and PLC β2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLCB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLC β2 HDR Plasmid (h) and PLC β2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLCB2 homology arms to support homology-directed repair at defined PLCB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.