PKC lambda/iota Double Nickase Plasmid (h): sc-402111-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKC lambda/iota Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PKC lambda/iota Double Nickase Plasmid (h) and PKC lambda/iota Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRKCI. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PKC lambda/iota Antibody (E-7): sc-376344
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC lambda/iota Double Nickase Plasmid (h) | sc-402111-NIC | 20 µg | $410.00 | |||
PKC lambda/iota Double Nickase Plasmid (h2) | sc-402111-NIC-2 | 20 µg | $410.00 |
PRKCI encodes the atypical protein kinase C isoform PKC lambda/iota (PKCι), a serine/threonine kinase that functions as a core component of the PAR polarity complex with PAR3 and PAR6. PKC lambda/iota integrates signals from Rho-family GTPases and growth factor pathways to regulate apical–basal polarity, tight junction assembly, asymmetric cell division, and directional migration. Through phosphorylation of polarity and trafficking substrates, it influences cytoskeletal remodeling and vesicle dynamics that shape epithelial organization. Dysregulated PRKCI/PKC lambda/iota signaling has been associated with altered polarity and invasive behavior in multiple tumor contexts, making it a useful node for mechanistic studies of oncogenic signaling and tissue architecture.
PKC lambda/iota Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRKCI locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRKCI. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRKCI function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRKCI-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.