PKC gamma Double Nickase Plasmid (h): sc-400503-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKC gamma Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PKC gamma Double Nickase Plasmid (h) and PKC gamma Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRKCG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PKC gamma Antibody (C-4): sc-166385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC gamma Double Nickase Plasmid (h) | sc-400503-NIC | 20 µg | $410.00 | |||
PKC gamma Double Nickase Plasmid (h2) | sc-400503-NIC-2 | 20 µg | $410.00 |
PRKCG encodes protein kinase C gamma (PKCγ), a neuron-enriched, Ca2+- and diacylglycerol-activated serine/threonine kinase that couples membrane signaling to phosphorylation-dependent control of excitability and synaptic function. PKCγ integrates upstream inputs from phospholipase C signaling to modulate cytoskeletal remodeling, vesicle trafficking, and activity-dependent transcription through pathways that intersect with MAPK/ERK and other kinase cascades. In human tissues, PRKCG is highly expressed in the central nervous system, where altered PKCγ signaling and coding variation have been associated with neurodegenerative and neurodevelopmental phenotypes. Experimental perturbation of PRKCG is commonly used to dissect signal transduction mechanisms governing neuronal plasticity, network activity, and phosphorylation-driven proteome changes.
PKC gamma Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRKCG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRKCG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRKCG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRKCG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.