Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PKC epsilon CRISPR/Cas9 KO Plasmid (h): sc-400358
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PKC epsilon CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PKC epsilon genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PKC epsilon Antibody (E-5): sc-1681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC epsilon CRISPR/Cas9 KO Plasmid (h) | sc-400358 | 20 µg | $397.00 |
Overview
PRKCE encodes protein kinase C epsilon (PKCε), a diacylglycerol-responsive serine/threonine kinase that transduces signals downstream of GPCR and receptor tyrosine kinase pathways. PKCε regulates phosphorylation networks controlling cell survival, proliferation, cytoskeletal remodeling, and directed migration, with prominent roles in MAPK/ERK, PI3K/AKT, NF-κB, and focal adhesion signaling. In human cells, PKCε also influences vesicle trafficking and mitochondrial stress responses, linking second-messenger signaling to metabolic adaptation. Dysregulated PRKCE/PKCε activity and expression have been associated with oncogenic phenotypes, therapy resistance mechanisms, and altered inflammatory signaling, making it a common target in pathway-mapping and functional genomics studies.
PKC epsilon CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRKCE gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRKCE together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRKCE open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PKC epsilon protein expression.
This CRISPR knockout system enables efficient generation of PRKCE-deficient cell models for investigation of PKC epsilon signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRKCE exon(s) critical for PKC epsilon function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRKCE genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PKC epsilon CRISPR/Cas9 KO Plasmid (h) and PKC epsilon CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRKCE locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PKC epsilon HDR Plasmid (h) and PKC epsilon HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRKCE homology arms to support homology-directed repair at defined PRKCE target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.