Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PELP1 CRISPR/Cas9 KO Plasmid (m): sc-429042
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PELP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PELP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PELP1 Antibody (E-1): sc-390599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PELP1 CRISPR/Cas9 KO Plasmid (m) | sc-429042 | 20 µg | $397.00 |
Overview
PELP1 (proline-, glutamic acid-, and leucine-rich protein 1), encoded by the mouse Pelp1 gene, is a multifunctional nuclear receptor coregulator and chromatin-associated scaffold that integrates steroid hormone signaling with transcriptional and epigenetic control. It coordinates interactions between estrogen receptor signaling and chromatin remodeling complexes, influencing RNA polymerase II–dependent transcription, histone modifications, and cell-cycle progression. PELP1 also interfaces with kinase pathways and DNA damage responses, linking growth factor cues to gene expression programs and genome maintenance. Dysregulated PELP1 activity and localization have been associated with aberrant proliferation, altered endocrine signaling, and oncogenic transcriptional networks, making it relevant for mechanistic studies of hormone-responsive biology and tumor-associated pathways.
PELP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pelp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pelp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pelp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PELP1 protein expression.
This CRISPR knockout system enables efficient generation of Pelp1-deficient cell models for investigation of PELP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pelp1 exon(s) critical for PELP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pelp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PELP1 CRISPR/Cas9 KO Plasmid (m) and PELP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pelp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PELP1 HDR Plasmid (m) and PELP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pelp1 homology arms to support homology-directed repair at defined Pelp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.