Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK2 Double Nickase Plasmid (h): sc-416916-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PDK2 Double Nickase Plasmid (h) and PDK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDK2 Antibody (S-15): sc-100534
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK2 Double Nickase Plasmid (h) | sc-416916-NIC | 20 µg | $410.00 |
Pyruvate dehydrogenase kinase 2 (PDK2) is a mitochondrial serine/threonine kinase that phosphorylates and inhibits the pyruvate dehydrogenase complex, thereby limiting conversion of pyruvate to acetyl-CoA and shifting carbon flux away from the TCA cycle. By regulating the pyruvate–lactate balance and oxidative metabolism, PDK2 integrates nutrient availability with mitochondrial respiration and lipid/glucose homeostasis. PDK2 activity is embedded in metabolic stress signaling and can influence reactive oxygen species handling and bioenergetic adaptation during hypoxia or nutrient excess. Dysregulated PDK2 expression or activity has been associated with altered insulin sensitivity, metabolic remodeling, and proliferative phenotypes observed across diverse disease contexts, supporting its use as a node to interrogate metabolic control mechanisms.
PDK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.