PDE6β Lentiviral Activation Particles (m): sc-422166-LAC
- Target species: mouse
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- PDE6β Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- PDE6β Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by PDE6β Lentiviral Activation Plasmid (m) and PDE6β Lentiviral Activation Plasmid (m2) target distinct regulatory regions of the Pde6b promoter. One or both designs may be available
- Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: PDE6β Antibody (B-8): sc-377486
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE6β Lentiviral Activation Particles (m) | sc-422166-LAC | 200 µl | $455.00 |
Mouse Pde6b encodes the β subunit of rod photoreceptor cGMP phosphodiesterase 6 (PDE6β), a core effector of the rhodopsin–transducin phototransduction cascade. Upon light-driven activation, PDE6β catalyzes cGMP hydrolysis, lowering intracellular cGMP to promote closure of cGMP-gated channels and shape the photoreceptor electrical response. This signaling axis governs cGMP and Ca²⁺ homeostasis in rod outer segments and integrates with downstream processes that influence photoreceptor survival and retinal circuit function. Disruption of PDE6β-dependent cGMP turnover is strongly linked to inherited retinal degeneration phenotypes, making Pde6b a key target for mechanistic studies of rod function and disease-associated signaling imbalance.
PDE6β Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Pde6b upregulation across a broader range of human cell types.
PDE6β Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Pde6b transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous PDE6β expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Pde6b genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.