Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PCAF Double Nickase Plasmid (h): sc-400753-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCAF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PCAF Double Nickase Plasmid (h) and PCAF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KAT2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PCAF Antibody (E-8): sc-13124
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCAF Double Nickase Plasmid (h) | sc-400753-NIC | 20 µg | $410.00 | |||
PCAF Double Nickase Plasmid (h2) | sc-400753-NIC-2 | 20 µg | $410.00 |
KAT2B encodes the histone acetyltransferase PCAF, a GNAT family coactivator that acetylates histones and non-histone substrates to regulate chromatin accessibility and transcriptional programs. PCAF participates in transcriptional control through interactions with multiprotein complexes and transcription factors, influencing processes such as DNA damage responses, cell-cycle progression, differentiation, and stress signaling. By modulating acetylation-dependent gene expression, PCAF contributes to pathways frequently altered in cancer and other disorders characterized by epigenetic dysregulation. Human PCAF is therefore a useful node for mechanistic studies linking chromatin remodeling to context-specific transcriptional outputs.
PCAF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KAT2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KAT2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KAT2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KAT2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.