CDKN1B/Kip1 p27 Antibody (F-8): sc-1641

p27 Kip1 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects p27 Kip1 of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). p27 Kip1 (F-8) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. p27 Kip1 plays a crucial role in regulating cell cycle progression by acting as a negative regulator of G1 phase transition, which is essential for maintaining proper cellular function and preventing uncontrolled cell proliferation. Elevated levels of p27 Kip1 are observed in response to DNA damage, particularly in a p53-dependent manner, highlighting its importance in cellular stress responses. p27 Kip1 interacts with D-type cyclins and cyclin-dependent kinase 4, as well as to a lesser extent with cyclin E and cyclin-dependent kinase 2, thereby influencing the activity of these key regulators in the cell cycle. p27 Kip1 modulation of cell cycle dynamics underscores its significance in cancer biology and therapeutic strategies targeting cell proliferation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CDKN1B/Kip1 p27 Antibody (F-8) References:

1. p27(Kip1) signaling: Transcriptional and post-translational regulation.  |  Hnit, SS., et al. 2015. Int J Biochem Cell Biol. 68: 9-14. PMID: 26279144
2. p27kip1 as a key regulator of endometriosis.  |  Gonçalves, GA. 2018. Eur J Obstet Gynecol Reprod Biol. 221: 1-4. PMID: 29216564
3. p27Kip1 regulates alpha-synuclein expression.  |  Gallastegui, E., et al. 2018. Oncotarget. 9: 16368-16379. PMID: 29662651
4. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.  |  Polyak, K., et al. 1994. Cell. 78: 59-66. PMID: 8033212
5. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21.  |  Toyoshima, H. and Hunter, T. 1994. Cell. 78: 67-74. PMID: 8033213
6. p15INK4B is a potential effector of TGF-beta-induced cell cycle arrest.  |  Hannon, GJ. and Beach, D. 1994. Nature. 371: 257-61. PMID: 8078588
7. A cell cycle-regulated inhibitor of cyclin-dependent kinases.  |  Hengst, L., et al. 1994. Proc Natl Acad Sci U S A. 91: 5291-5. PMID: 8202483
8. WAF1, a potential mediator of p53 tumor suppression.  |  el-Deiry, WS., et al. 1993. Cell. 75: 817-25. PMID: 8242752
9. p21 is a universal inhibitor of cyclin kinases.  |  Xiong, Y., et al. 1993. Nature. 366: 701-4. PMID: 8259214
10. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4.  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
11. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.  |  Polyak, K., et al. 1994. Genes Dev. 8: 9-22. PMID: 8288131
12. Mammalian G1 cyclins.  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CDKN1B/Kip1 p27 Antibody (F-8) has 673 citations in a variety of scientific publications.

Primary antibodies like Anti-CDKN1B/Kip1 p27 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CDKN1B/Kip1 p27 Antibody (F-8) Specifications

Alternate Product Name: p27 Kip1

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-197

Epitope Target: p27 Kip1

Epitope Species: mouse

Product Reactivity/Detection: p27 Kip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 673 citations in various scientific publications

Available Conjugates/Forms for CDKN1B/Kip1 p27 Antibody (F-8): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine