OX2 Antibody (OX90): sc-53100
- OX2 Antibody (OX90) is a rat monoclonal IgG2a OX2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against CD200 of mouse origin
- OX2 Antibody (OX90) is recommended for detection of OX2 cell surface antigen of mouse origin by WB, IP, IF and FCM
- Anti-OX2 Antibody (OX90) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for OX2 Antibody (OX90). This work is in progress.
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OX2 Antibody (OX90) is a rat monoclonal IgG2a antibody that detects OX2 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-OX2 antibody (OX90) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OX2, also known as CD200 or MOX2, is a 248-residue cell surface glycoprotein that plays a crucial role in immune regulation by interacting with OX2R. This interaction helps modulate immune responses, potentially preventing excessive inflammation and tissue damage. OX2 is predominantly expressed in lymphoid cells, neurons, and endothelial cells, while OX2R is primarily found in lymphoid and neuronal tissues. OX2R includes a significant portion of its weight derived from N-linked glycosylation, which can influence receptor stability, localization, and interaction with ligands. OX2R′s cytoplasmic domain contains NPXY motifs that facilitate interactions with specific binding domains, further underscoring structural features′ importance in mediating cellular signaling pathways. OX2 and OX2R interaction plays a vital role in suppressing macrophage-induced inflammatory damage, highlighting OX2′s significance in maintaining immune homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
OX2 Antibody (OX90) References:
- Differential distribution and regulation of OX1 and OX2 orexin/hypocretin receptor messenger RNA in the brain upon fasting. | Lu, XY., et al. 2000. Horm Behav. 37: 335-44. PMID: 10860677
- Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. | Wright, GJ., et al. 2000. Immunity. 13: 233-42. PMID: 10981966
- Receptor engagement on cells expressing a ligand for the tolerance-inducing molecule OX2 induces an immunoregulatory population that inhibits alloreactivity in vitro and in vivo. | Gorczynski, RM., et al. 2000. J Immunol. 165: 4854-60. PMID: 11046009
- Down-regulation of the macrophage lineage through interaction with OX2 (CD200). | Hoek, RM., et al. 2000. Science. 290: 1768-71. PMID: 11099416
- Distribution of OX2 antigen and OX2 receptor within retina. | Dick, AD., et al. 2001. Invest Ophthalmol Vis Sci. 42: 170-6. PMID: 11133863
- Putting the brakes on innate immunity: a regulatory role for CD200? | Nathan, C. and Muller, WA. 2001. Nat Immunol. 2: 17-9. PMID: 11135572
- Constitutive retinal CD200 expression regulates resident microglia and activation state of inflammatory cells during experimental autoimmune uveoretinitis. | Broderick, C., et al. 2002. Am J Pathol. 161: 1669-77. PMID: 12414514
- Orexins/hypocretins modulate the activity of NPY-positive and -negative neurons in the rat intergeniculate leaflet via OX1 and OX2 receptors. | Palus, K., et al. 2015. Neuroscience. 300: 370-80. PMID: 26022361
- Functional interaction between OX2 and CB1 receptors in the ventral tegmental area and the nucleus accumbens in response to place preference induced by chemical stimulation of the lateral hypothalamus. | Yazdi, F., et al. 2015. Pharmacol Biochem Behav. 139: 39-46. PMID: 26494513
- Sequential expression of OX2 and Thy-1 glycoproteins on the neuronal surface during development. An immunohistochemical study of rat cerebellum. | Morris, RJ. and Beech, JN. 1987. Dev Neurosci. 9: 33-44. PMID: 2885171
- Identification of Ia glycoproteins in rat thymus and purification from rat spleen. | McMaster, WR. and Williams, AF. 1979. Eur J Immunol. 9: 426-33. PMID: 315315
- The gene for MRC OX-2 membrane glycoprotein is localized on human chromosome 3. | McCaughan, GW., et al. 1987. Immunogenetics. 25: 133-5. PMID: 3817906
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OX2 Antibody (OX90) | sc-53100 | 200 µg/ml | $322.00 | |||
OX2 Antibody (OX90) AC | sc-53100 AC | 500 µg/ml, 25% agarose | $424.00 | |||
OX2 Antibody (OX90) HRP | sc-53100 HRP | 200 µg/ml | $322.00 | |||
OX2 Antibody (OX90) FITC | sc-53100 FITC | 200 µg/ml | $336.00 | |||
OX2 Antibody (OX90) PE | sc-53100 PE | 200 µg/ml | $349.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 488 | sc-53100 AF488 | 200 µg/ml | $364.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 546 | sc-53100 AF546 | 200 µg/ml | $364.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 594 | sc-53100 AF594 | 200 µg/ml | $364.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 647 | sc-53100 AF647 | 200 µg/ml | $364.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 680 | sc-53100 AF680 | 200 µg/ml | $364.00 | |||
OX2 Antibody (OX90) Alexa Fluor® 790 | sc-53100 AF790 | 200 µg/ml | $364.00 |